Used in research settings to quantify levels of AANAT enzyme activity in mouse tissue samples, particularly in studies related to circadian rhythm regulation and melatonin synthesis. The kit enables precise measurement of AANAT protein concentration in pineal gland extracts or other biological samples, supporting investigations into sleep disorders, seasonal affective conditions, and neuroendocrine function. It is not intended for diagnostic use, but serves as a critical tool in preclinical and basic science research involving rodent models.