Used in biomedical research to quantify 5-nucleotidase enzyme levels in biological samples, aiding in the study of cellular metabolism, immune function, and liver health. Commonly applied in immunology and clinical laboratories for enzyme activity assessment through ELISA methodology. Supports research into purinergic signaling pathways and can be used to investigate conditions involving ectoenzyme dysregulation, such as inflammation, cancer, and metabolic disorders. Suitable for use with serum, plasma, and cell culture supernatants.