Used in biomedical research to measure NADPH oxidase activity in rat tissue samples, particularly in studies related to oxidative stress and inflammation. Helps researchers evaluate the production of reactive oxygen species (ROS) in various disease models such as cardiovascular disorders, neurodegenerative diseases, and chronic kidney disease. Commonly applied in pharmacological studies to assess the effectiveness of antioxidant treatments or anti-inflammatory drugs. Enables quantitative analysis of enzyme levels in cell lysates and tissue homogenates, supporting mechanistic studies in signal transduction and immune response pathways.