Used in biomedical research to quantify levels of cytochrome P450 3A4 enzyme in mouse samples. Enables assessment of drug metabolism, liver function, and enzyme induction or inhibition in preclinical studies. Widely applied in pharmaceutical development to evaluate drug candidates' effects on metabolic pathways. Supports research on drug-drug interactions, toxicology, and regulation of CYP450 enzymes in experimental models. Provides a reliable, sensitive, and specific method for high-throughput screening in rodent-based studies.