Used in biomedical research to measure proline hydroxylase enzyme activity, particularly in studies related to hypoxia and oxygen sensing pathways. Helps researchers investigate conditions such as cancer, ischemia, and anemia by quantifying PHD expression levels in cell or tissue samples. Commonly applied in drug development to assess the effectiveness of HIF (hypoxia-inducible factor) stabilizers or PHD inhibitors. Provides a reliable, high-throughput method for detecting PHD protein concentration in experimental models.