Used in biomedical research to quantify levels of the PFKFB3 enzyme in human tissue extracts and biological fluids. This assay helps investigate metabolic pathways linked to glycolysis regulation, particularly in cancer cells where PFKFB3 is often overexpressed. Researchers use the kit to assess the enzyme’s role in tumor progression, inflammation, and cellular responses to hypoxia. It is also applied in drug development studies to evaluate the effectiveness of metabolic inhibitors targeting cancer and other diseases involving dysregulated glucose metabolism.