Used in biomedical research to quantify levels of 6-phosphofructo-1-kinase (PFK-1) in human samples. This enzyme is a key regulator of glycolysis, so the kit helps study metabolic disorders, diabetes, and cancer metabolism. Researchers use it to assess PFK-1 activity indirectly by measuring protein concentration in cell lysates or tissue extracts. It supports investigations into insulin signaling, glucose metabolism, and mitochondrial function. Commonly applied in clinical labs and pharmaceutical research to evaluate metabolic pathway alterations in disease states.