Used in biomedical research to quantify TRACP-2b levels in biological samples such as serum or plasma. Helps assess bone resorption activity, particularly in studies related to osteoporosis, Paget’s disease, and bone metastasis. Widely applied in clinical research settings to monitor osteoclast function and evaluate the effectiveness of anti-resorptive therapies like bisphosphonates or denosumab. Also used in inflammatory and autoimmune disease research due to the enzyme's presence in activated macrophages. Provides a reliable, high-throughput method for detecting TRACP-2b with high sensitivity and specificity.