Used in biomedical research to detect and quantify deubiquitinating enzyme activity in human samples. Helps study the ubiquitin-proteasome system (UPS), which plays a key role in protein degradation, cell cycle regulation, DNA repair, and immune response. Applied in investigating neurodegenerative diseases, cancer, and inflammatory conditions where UPS dysfunction is implicated. Enables researchers to assess changes in deubiquitinase levels under experimental treatments, supporting drug development and mechanistic studies.