Used in plant biology research to quantify vacuolar H+-ATPase (V-ATPase) activity in cellular and subcellular fractions. This ELISA kit enables researchers to measure changes in V-ATPase protein levels under different physiological conditions such as abiotic stress, nutrient deficiency, or pathogen attack. It supports studies on intracellular pH regulation, ion transport, and organelle acidification in plant cells. Commonly applied in experiments involving root development, stomatal regulation, and responses to salinity or heavy metals. Provides a reliable, high-throughput method for protein detection without requiring radioisotopes or complex instrumentation.