Soil Leucine Aminopeptidase(S-LAP) Activity Assay Kit
Spectrophotometry
Reagent
Code: #67056
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Storage & Handling
Storage
2-8℃, avoid light
description Product Description
The Soil Leucine Aminopeptidase (S-LAP) Activity Assay Kit is primarily used to measure the activity of leucine aminopeptidase in soil samples. This enzyme plays a critical role in soil nitrogen cycling by breaking down proteins and peptides into amino acids, which are essential nutrients for plants and microorganisms.
In agricultural research, the kit helps assess soil health and fertility by evaluating the enzymatic activity that influences nutrient availability. It is also utilized in environmental studies to monitor the impact of land management practices, pollution, or climate change on soil microbial activity. Additionally, the kit aids in bioremediation research by measuring the degradation of organic pollutants in contaminated soils.
Its application extends to ecological studies, where it provides insights into microbial community dynamics and their role in ecosystem functioning. By quantifying S-LAP activity, researchers can better understand soil nutrient turnover and develop strategies for sustainable soil management.
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| Test Parameter | Specification |
|---|---|
| Performance test | PASS |
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Soil Leucine Aminopeptidase (S-LAP) Activity Assay Kit
Product code: 67056
Method: Spectrophotometric method
Size: 50T
Product Introduction
Soil leucine aminopeptidase (S-LAP) is a class of enzymes secreted by soil microorganisms that hydrolyze peptide chains with N-terminal leucine. Changes in S-LAP activity are closely related to certain pathological states of the body.
S-LAP breaks down L-leucyl-p-nitroanilide to produce p-nitroaniline, which has a maximum absorption peak at 405 nm. S-LAP activity is calculated by measuring the rate of increase in absorbance.
Package Contents
| Code | Component | Quantity |
|---|---|---|
| 67056.1 | Reagent I | 1 bottle |
| 67056.2 | Reagent II | 1 bottle |
| 67056.m | Instructions | 1 copy |
Quality Standards and Safety Information
| Raw Material or Packaging Name | Quality Standard | Main Toxicity |
|---|---|---|
| Reagent I | -- | -- |
| Reagent II | -- | -- |
Transportation and Storage
| Transportation | Transport with ice packs. |
|---|---|
| Storage | Store Reagent I at 4℃ and Reagent II at -20℃. |
| Shelf Life | 180 days |
Instructions for Use
1. Reagent Preparation
- Add 30 mL of Reagent I to the Reagent II bottle and dissolve thoroughly.
- If dissolution is difficult, heat in a 60℃ water bath for about 30 min to promote dissolution.
- Place the prepared reagent in a 37℃ water bath for mammals, or a 25℃ water bath for other species, for 10 min or longer before use.
- Aliquot any unused reagent and store at -20℃. Do not freeze and thaw repeatedly.
2. Assay Procedure
- Preheat the spectrophotometer for 30 min or longer. Set the wavelength to 405 nm and zero with distilled water.
- Add samples according to the table below.
| Component | Control Tube | Assay Tube |
|---|---|---|
| Fresh soil sample (g) | 0.2 | 0.2 |
| Reagent I (μL) | 1200 | - |
| Reagent II (μL) | - | 1200 |
- Mix well and incubate with shaking at 37℃ for 1 h.
- Centrifuge at 8000 g and 4℃ for 10 min.
- Transfer 1 mL of supernatant into a cuvette and measure the absorbance at 405 nm.
- Calculate ΔA = Aassay- Acontrol. Set one control tube for each assay tube.
3. S-LAP Activity Calculation
Unit definition: The amount of enzyme in 1 g soil sample that generates 1 μmol p-nitroaniline per day is defined as one unit of enzyme activity.
S-LAP activity (μmol/d·g-1, soil sample) = ΔA × Vtotal reaction÷ (ε × d) × 106÷ W ÷ T = 14.8 × ΔA
| Symbol | Description | Value |
|---|---|---|
| Vtotal reaction | Total volume of the reaction system | 1.2 × 10-3L |
| ε | Molar extinction coefficient of p-nitroaniline | 9.72 × 103L/mol·cm-1 |
| d | Optical path length of the cuvette | 1 cm |
| T | Reaction time | 1 h = 1/24 d |
| W | Sample mass | 0.2 g |
Precautions
- This 50T kit can test 24 samples.
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