UV-VIS (IC50) Anti-Elastase Assay

UV-VIS (IC50) Anti-Elastase Assay
  • Product Code: 253558
UV-VIS (IC50) Anti-Elastase Assay
฿6,000.00

science Sample Names

assignment Instructions

info Please include any specific requirements or conditions for testing

description Service Overview

UV-VIS (IC50) Anti-Elastase Assay

timeline Service Steps

Step Procedure Expected Result
1
Prepare 0.2...
check_circle_outline Clear colorless buffer.
2
Prepare porcine...
check_circle_outline Clear enzyme solution.
3
Prepare substrate...
check_circle_outline Clear yellow-tinged substrate solution.
4
Dissolve sample...
check_circle_outline Clear dilution series; no precipitation.
5
In 96-well...
check_circle_outline Plate ready for substrate addition.
6
Add 100...
check_circle_outline Yellow color development in control wells.
7
Read absorbance...
check_circle_outline Raw absorbance data (control ~0.5-1.0 AU).
8
Calculate %...
check_circle_outline IC50 value with 95% CI.
UV-VIS (IC50) Anti-Elastase Assay
UV-VIS (IC50) Anti-Elastase Assay
UV-VIS (IC50) Anti-Elastase Assay
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Service Steps
Step Procedure Expected Result
1 Prepare 0.2... Clear colorless buffer.
2 Prepare porcine... Clear enzyme solution.
3 Prepare substrate... Clear yellow-tinged substrate solution.
4 Dissolve sample... Clear dilution series; no precipitation.
5 In 96-well... Plate ready for substrate addition.
6 Add 100... Yellow color development in control wells.
7 Read absorbance... Raw absorbance data (control ~0.5-1.0 AU).
8 Calculate %... IC50 value with 95% CI.