Used in research to inhibit the EZH2 and EZH1 enzymes, which play roles in gene regulation through histone methylation. This compound helps study epigenetic mechanisms, particularly in cancer biology. It shows potential in investigating hematological cancers like lymphoma and leukemia, where EZH2 mutations are common. Due to its ability to cross the blood-brain barrier, it is also explored in studies of brain tumors and neurodevelopmental disorders linked to epigenetic dysregulation. Primarily used in preclinical research to understand tumor growth, cell differentiation, and chromatin dynamics.