LDH-IN-1 is primarily used in research settings to study the role of lactate dehydrogenase (LDH) in various biological processes. It serves as an inhibitor of LDH, making it a valuable tool for investigating metabolic pathways, particularly those involving glycolysis and anaerobic respiration. Researchers utilize LDH-IN-1 to explore its potential in targeting cancer cells, as many tumors rely on heightened LDH activity for energy production. Additionally, it is employed in studies related to ischemia-reperfusion injury, where LDH activity plays a critical role in tissue damage. Its application extends to understanding the mechanisms of diseases associated with metabolic dysregulation, such as neurodegenerative disorders and cardiovascular conditions.