LDHA-IN-3 is primarily utilized in the field of cancer research due to its ability to inhibit lactate dehydrogenase A (LDHA), an enzyme that plays a critical role in the metabolism of cancer cells. By targeting LDHA, this compound disrupts the anaerobic glycolysis pathway, which is essential for the survival and proliferation of many cancer cells, particularly in hypoxic (low oxygen) environments. This makes it a promising candidate for the development of anticancer therapies, as it can potentially reduce tumor growth and metastasis. Additionally, LDHA-IN-3 is used in biochemical studies to better understand the metabolic reprogramming in cancer cells and to explore the therapeutic potential of LDHA inhibition in various cancer types. Its application extends to preclinical studies where it helps in evaluating the efficacy and safety of LDHA-targeted treatments.