Nor-NOHA monoacetate is primarily used in biochemical research to study the role of arginase enzymes in various physiological and pathological processes. It serves as a potent inhibitor of arginase, an enzyme that plays a critical role in the urea cycle and nitric oxide (NO) production. By inhibiting arginase, this compound helps researchers understand its impact on cellular metabolism, immune response, and vascular function. It is particularly valuable in studies related to cardiovascular diseases, pulmonary hypertension, and cancer, where arginase activity is often dysregulated. Additionally, nor-NOHA monoacetate is utilized in drug development to explore potential therapeutic strategies targeting arginase pathways. Its application extends to investigating the interplay between arginase and nitric oxide synthase (NOS) in conditions like inflammation, fibrosis, and endothelial dysfunction.