PGAM1-IN-2 is primarily utilized in research settings to study its inhibitory effects on phosphoglycerate mutase 1 (PGAM1), an enzyme involved in glycolysis and biosynthesis pathways. By targeting PGAM1, this compound helps researchers explore its potential role in regulating cancer cell metabolism, as cancer cells often rely on altered metabolic pathways for growth and survival. It serves as a tool to investigate the enzyme's function in tumor progression and to assess the therapeutic potential of PGAM1 inhibition in cancer treatment. Additionally, PGAM1-IN-2 is used in biochemical studies to understand metabolic reprogramming in various diseases, providing insights into the development of targeted therapies.