ThrRS-IN-2 is primarily used in research settings to study bacterial protein synthesis and develop new antibiotics. It specifically targets threonyl-tRNA synthetase (ThrRS), an enzyme crucial for protein production in bacteria. By inhibiting this enzyme, it disrupts bacterial growth, making it a potential candidate for combating antibiotic-resistant infections. Researchers also utilize it to explore the mechanisms of enzyme inhibition and its implications in bacterial survival. This compound aids in understanding the structure and function of ThrRS, which is essential for designing more effective antimicrobial agents. Its application is significant in the ongoing effort to address the global challenge of drug-resistant bacterial strains.