SKF-34288 hydrochloride, also known as 3-Mercaptopicolinic acid, is primarily utilized in biochemical research as a potent inhibitor of gluconeogenesis. It specifically targets the enzyme phosphoenolpyruvate carboxykinase (PEPCK), which plays a critical role in the metabolic pathway that converts non-carbohydrate substrates into glucose. By inhibiting PEPCK, this compound helps researchers study the regulation of glucose production in the liver, providing insights into metabolic disorders such as diabetes and obesity. Additionally, it is used in experimental settings to explore the effects of reduced gluconeogenesis on energy metabolism and to evaluate potential therapeutic strategies for managing hyperglycemia. Its application extends to pharmacological studies where it aids in understanding the mechanisms of enzyme inhibition and metabolic control.