RNA Polymerase III Inhibitor is primarily used in research to study the mechanisms of transcription and gene expression. By inhibiting RNA Polymerase III, researchers can investigate the role of this enzyme in the synthesis of small RNAs, such as tRNAs and 5S rRNA, which are crucial for protein synthesis. This inhibitor is also employed in cancer research to explore the potential of targeting RNA Polymerase III as a therapeutic strategy, given its role in cell growth and proliferation. Additionally, it helps in understanding the regulatory pathways involved in cellular stress responses and metabolic processes.