4-Deoxypyridoxine 5'-phosphate is used primarily in biochemical and pharmacological research to understand the mechanisms of vitamin B6 and related enzymes. Due to its structural similarity to pyridoxal 5'-phosphate (PLP), a key cofactor in amino acid metabolism, it acts as an inhibitor to study the effects on PLP-dependent reactions and elucidate enzyme mechanisms in metabolic pathways. It is also employed in investigating vitamin B6 deficiency and its effects on cellular processes. Additionally, studies explore its role in inhibiting the growth of certain cancer cells by disrupting DNA synthesis and other cell division processes. In medical research, it serves as a tool to explore potential therapeutic targets for conditions linked to pyridoxine metabolism disorders.