DL-Fluorocitric acid barium salt is primarily utilized in biochemical research as a potent inhibitor of aconitase, an enzyme involved in the tricarboxylic acid (TCA) cycle. By inhibiting aconitase, it disrupts cellular energy production, making it a valuable tool for studying metabolic pathways and cellular respiration. It is also employed in experimental studies to investigate the effects of metabolic inhibition on cell function, particularly in cancer research, where altered metabolism is a key feature. Additionally, it serves as a reference compound in analytical chemistry for method development and validation in detecting fluorocitrate derivatives.