CCT0129397 is primarily used in biochemical and pharmacological research as a selective inhibitor of the protein-protein interaction between 14-3-3 and specific client proteins. It has been shown to disrupt 14-3-3/signal transducer interactions, making it a valuable tool for studying cellular signaling pathways involved in cancer, neurodegenerative diseases, and apoptosis. In cancer research, CCT0129397 has demonstrated the ability to reactivate pro-apoptotic proteins that are sequestered by 14-3-3, thereby promoting cancer cell death. Its application extends to studies exploring targeted therapies where restoring normal regulatory mechanisms in diseased cells is a goal. Due to its cell permeability and specificity, it is widely used in cell-based assays to probe 14-3-3-dependent biological processes.