TC HSD 21 is primarily used in research and development within the pharmaceutical and biochemical fields. It serves as a key compound in studies related to steroidogenesis, particularly in understanding the enzymatic pathways involved in the biosynthesis of steroid hormones. Researchers utilize it to investigate the role of specific enzymes in metabolic processes, which can lead to the development of targeted therapies for endocrine disorders. Additionally, it is employed in the synthesis of steroid analogs, which are crucial for creating potential drug candidates with improved efficacy and reduced side effects. Its application extends to biochemical assays where it acts as a substrate or inhibitor to study enzyme kinetics and mechanisms, contributing to advancements in both basic science and therapeutic innovation.