Exo1 is widely utilized in molecular biology and genetic research due to its role in DNA repair and recombination. It is an exonuclease that processes DNA ends, making it essential for studies involving DNA mismatch repair and homologous recombination. Researchers employ Exo1 to generate single-stranded DNA overhangs, which are crucial for various experimental techniques, including DNA sequencing, cloning, and genome editing. Additionally, its involvement in maintaining genomic stability makes it a valuable tool for investigating mechanisms of cancer and other diseases linked to DNA repair deficiencies. Exo1 is also used in vitro to study the dynamics of DNA repair pathways and to develop assays for screening potential therapeutic compounds targeting DNA repair processes.