Phenylalanine ammonia-lyase from Rhodotorula glutinis is widely used in the food industry for the production of low-phenylalanine foods, catering to individuals with phenylketonuria (PKU). This enzyme helps in breaking down phenylalanine, making it easier to create dietary products safe for PKU patients. In the pharmaceutical sector, it plays a crucial role in synthesizing L-phenylalanine derivatives, which are essential in the production of various drugs and therapeutic agents. Additionally, it is utilized in biotechnological research for studying enzyme kinetics and metabolic pathways related to phenylalanine metabolism. The enzyme also finds application in the development of biosensors designed to detect phenylalanine levels in biological samples, aiding in the diagnosis and management of metabolic disorders. Its ability to catalyze specific reactions makes it valuable in industrial biotechnology for producing fine chemicals and intermediates.