Used in molecular biology for dephosphorylation of DNA and RNA to prevent self-ligation during cloning. Commonly applied in restriction enzyme cloning and vector preparation. Also used in diagnostics as a label in immunoassays, especially in ELISA, to detect biomarkers through colorimetric, chemiluminescent, or fluorescent signals. In clinical chemistry, its enzymatic activity is measured to assess liver and bone disorders. Employed in research to study phosphorylation states and enzyme kinetics. Stable and easy to detect, making it ideal for histochemical staining to visualize tissue enzyme activity.