Used in biotechnological processes for the production of organic acids and amino acids by catalyzing the fixation of carbon dioxide into oxaloacetate. Plays a key role in metabolic engineering of microorganisms to enhance carbon flux in pathways for biosynthesis of chemicals like malate, aspartate, and aromatic compounds. Applied in research on carbon concentration mechanisms and improvement of microbial CO2 fixation efficiency. Also utilized in enzymatic assays for studying metabolic regulation in microbial systems.