Used in molecular biology for precise DNA digestion, particularly in restriction analysis and cloning workflows. Its high specificity allows clean cutting at unique recognition sites, minimizing star activity. Commonly applied in genotyping, plasmid mapping, and verification of recombinant constructs. The high concentration (20U/μL) supports efficient digestion with small reaction volumes, ideal for high-throughput applications. Stable under standard buffer conditions, it enables double digestions with other enzymes without requiring additional buffer optimization.