Butyrylcholinesterase from equine serum is widely used in biochemical research to study enzyme kinetics and inhibition. It serves as a model enzyme for understanding the mechanisms of cholinesterase activity, which is crucial in developing treatments for neurodegenerative diseases like Alzheimer's. Additionally, it is employed in toxicology studies to assess the effects of organophosphate and carbamate pesticides, as these compounds inhibit cholinesterase activity. The enzyme is also utilized in forensic science to detect and quantify exposure to nerve agents. In biotechnology, it aids in the development of biosensors for detecting cholinesterase inhibitors in environmental and biological samples. Its application extends to pharmacology, where it helps in screening potential therapeutic agents targeting cholinesterase-related disorders.