Glyoxalase I, derived from Saccharomyces cerevisiae, is primarily utilized in biochemical research to study the glyoxalase system, which plays a critical role in detoxifying methylglyoxal, a byproduct of glycolysis. This enzyme is essential in understanding cellular mechanisms related to oxidative stress and metabolic regulation. It is also employed in enzymatic assays to investigate the pathways involved in the detoxification process, providing insights into diseases linked to oxidative damage, such as diabetes and neurodegenerative disorders. Additionally, Glyoxalase I is used in the development of potential therapeutic strategies targeting metabolic disorders and aging-related conditions.