Citrate lyase from Klebsiella pneumoniae is widely utilized in biochemical research and industrial applications. It plays a crucial role in the enzymatic breakdown of citrate into oxaloacetate and acetate, making it valuable in metabolic pathway studies. In the food industry, it is employed to enhance flavor profiles by modulating citrate levels in products like dairy and beverages. Additionally, it finds use in diagnostic kits for detecting citrate in biological samples, aiding in the diagnosis of metabolic disorders. Its application in biotechnology includes the production of organic acids and the development of biosensors for citrate detection.