Alkaline phosphatase is widely used in molecular biology for the dephosphorylation of DNA, RNA, and proteins, which is essential for preventing self-ligation in cloning experiments. In clinical diagnostics, it serves as a key enzyme in various immunoassays and biochemical tests, particularly in the detection of liver and bone disorders. The enzyme is also employed in histochemistry to identify specific tissues and cells by catalyzing colorimetric reactions. Additionally, it plays a role in biomanufacturing processes, such as the production of monoclonal antibodies, where it helps in the removal of phosphate groups from molecules. Its application extends to environmental monitoring, where it is used as a biomarker to assess water quality and soil health.