Used primarily in clinical diagnostics as part of enzymatic assays to indirectly measure creatinine levels in blood and urine (through conversion to creatine), aiding in the assessment of kidney function, while also enabling direct measurement of creatine levels related to muscle disorders. It is employed in biochemical research to study creatine metabolism and related pathways. In the food industry, it helps in the development and quality control of nutritional supplements aimed at enhancing athletic performance by optimizing creatine utilization. Additionally, it plays a role in various enzymatic assays for detecting and quantifying creatine in biological samples.