Thymidine Phosphorylase, recombinant, from Escherichia coli, is widely utilized in biochemical research and pharmaceutical development. It plays a critical role in the study of nucleoside metabolism, particularly in the conversion of thymidine to thymine and 2-deoxy-D-ribose-1-phosphate. This enzyme is essential in the development of anticancer drugs, as it is involved in the activation of certain prodrugs that target tumor cells. Additionally, it is used in enzymatic assays to measure thymidine levels in biological samples, aiding in the understanding of cellular processes and DNA synthesis. Its recombinant form ensures high purity and consistency, making it a valuable tool in both academic and industrial laboratories.