Naphthol AS-D chloroacetate is widely used in histochemistry as a substrate for the detection of esterase activity. It is particularly valuable in identifying specific enzymes in tissue samples, aiding in the diagnosis and research of various biological and pathological conditions. When the compound is hydrolyzed by esterases, it produces a colored precipitate, allowing for the visualization of enzyme activity under a microscope. This makes it an essential tool in laboratories for studying cellular functions, enzyme localization, and tissue integrity. Its specificity and reliability have made it a preferred choice in diagnostic and research applications.