Methyl trans-3-Pentenoate is primarily used in the synthesis of flavors and fragrances due to its pleasant ester-like aroma. It serves as a key intermediate in the production of various organic compounds, particularly in the development of fruity and floral scent profiles for perfumes, cosmetics, and food flavorings. Additionally, it is employed in the pharmaceutical industry as a building block for the synthesis of more complex molecules, contributing to the creation of active pharmaceutical ingredients (APIs). Its reactivity and structure make it a versatile compound in organic chemistry research and industrial applications.