Used primarily in research to study cholesterol metabolism and trafficking within cells. It inhibits cholesterol transport, making it valuable for understanding Niemann-Pick Type C disease, a condition characterized by cholesterol accumulation in lysosomes. Also employed in lipid biology studies to investigate lipid droplet formation and autophagy processes. Its ability to disrupt cholesterol homeostasis aids in exploring the role of cholesterol in neurodegenerative diseases and cellular signaling pathways.