(±)4-HDHA is primarily used in research and development within the field of biochemistry and pharmacology. It serves as a key intermediate in the synthesis of bioactive lipid mediators, which play a significant role in inflammation and immune response regulation. This compound is particularly valuable in studying the pathways and mechanisms of omega-3 fatty acid metabolism, as it is involved in the production of specialized pro-resolving mediators (SPMs) that help resolve inflammation and promote tissue repair. Additionally, (±)4-HDHA is utilized in the development of therapeutic agents aimed at treating chronic inflammatory diseases, cardiovascular conditions, and neurodegenerative disorders. Its application extends to the formulation of dietary supplements designed to enhance anti-inflammatory and neuroprotective effects.