Octanoyl-L-carnitine chloride is primarily used in research and biomedical applications due to its role in lipid metabolism and energy production. It serves as a key compound in studying mitochondrial function, as it facilitates the transport of fatty acids into mitochondria for beta-oxidation. This makes it valuable in understanding metabolic disorders, such as fatty acid oxidation defects. Additionally, it is utilized in the development of therapeutic strategies for conditions like obesity, diabetes, and cardiovascular diseases. In biochemical assays, it acts as a substrate or intermediate to investigate enzyme activity related to carnitine acyltransferases. Its applications also extend to nutritional science, where it is explored for its potential in enhancing energy metabolism and physical performance.