Used in biochemical research to study metabolic pathways, particularly those involving fatty acid metabolism. It serves as a substrate or intermediate in enzymatic reactions, helping to understand the mechanisms of enzymes like acyl-CoA dehydrogenases. Applied in vitro to investigate lipid biosynthesis and degradation processes. Also utilized in the development of assays to detect enzyme activity or screen potential inhibitors for therapeutic purposes. Its role in energy production and cellular metabolism makes it valuable in studies related to metabolic disorders and diseases.