Acetoacetyl coenzyme A sodium salt hydrate is widely used in biochemical research and enzymatic studies. It serves as a key intermediate in metabolic pathways, particularly in the biosynthesis of ketone bodies and fatty acids. Researchers utilize it to investigate the mechanisms of enzymes like thiolase and HMG-CoA synthase, which are crucial in lipid metabolism. It is also employed in the study of cellular energy production and the regulation of metabolic processes. Additionally, this compound is used in the development of assays to measure enzyme activity and to explore metabolic disorders related to lipid and energy metabolism. Its role in synthetic biology and metabolic engineering further highlights its importance in producing biofuels and other bioproducts.