Sheath Fluid (1×) is primarily used in flow cytometry to maintain the stability and alignment of cells as they pass through the flow cytometer. It ensures that cells are uniformly suspended and properly focused in a single-file stream, which is critical for accurate analysis and sorting of cells based on their size, granularity, and fluorescence properties. This fluid also prevents clogging and maintains the integrity of the instrument’s fluidics system, enabling consistent and reliable results in applications such as immunophenotyping, cell cycle analysis, and apoptosis studies. Additionally, it is compatible with a wide range of biological samples, making it a versatile component in cell-based research and diagnostics.