Fluorescein Biotin
98%
Reagent
Code: #108307
CAS Number
134759-22-1
blur_circular Chemical Specifications
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Molecular Information
Weight
831.01 g/mol
Formula
C₄₂H₅₀N₆O₈S₂
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Storage & Handling
Storage
-20°C, airtight, dry
description Product Description
Fluorescein biotin is widely used in molecular biology and biochemistry for labeling and detection purposes. Its applications include:
- **Fluorescent Labeling**: It is commonly used to label proteins, nucleic acids, and other biomolecules for visualization under a fluorescence microscope. The fluorescein moiety provides a strong green fluorescence signal, making it ideal for tracking and imaging.
- **Biotin-Streptavidin System**: The biotin component allows for strong and specific binding to streptavidin or avidin, enabling its use in affinity purification, immunoassays, and diagnostic techniques like ELISA.
- **Flow Cytometry**: It is employed in flow cytometry to tag cells or particles, facilitating the analysis and sorting of specific populations based on fluorescence.
- **In Situ Hybridization**: Fluorescein biotin is used in fluorescence in situ hybridization (FISH) to label DNA or RNA probes, aiding in the detection of specific genetic sequences within cells or tissues.
- **Biosensors**: It is integrated into biosensor designs for detecting biomolecular interactions, leveraging its fluorescence for real-time monitoring.
These applications make it a versatile tool in research, diagnostics, and biotechnology.
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Fluorescein Biotin
Fluorescein biotin is widely used in molecular biology and biochemistry for labeling and detection purposes. Its applications include:
- **Fluorescent Labeling**: It is commonly used to label proteins, nucleic acids, and other biomolecules for visualization under a fluorescence microscope. The fluorescein moiety provides a strong green fluorescence signal, making it ideal for tracking and imaging.
- **Biotin-Streptavidin System**: The biotin component allows for strong and specific binding to streptavidin or avidin, enabling its use in affinity purification, immunoassays, and diagnostic techniques like ELISA.
- **Flow Cytometry**: It is employed in flow cytometry to tag cells or particles, facilitating the analysis and sorting of specific populations based on fluorescence.
- **In Situ Hybridization**: Fluorescein biotin is used in fluorescence in situ hybridization (FISH) to label DNA or RNA probes, aiding in the detection of specific genetic sequences within cells or tissues.
- **Biosensors**: It is integrated into biosensor designs for detecting biomolecular interactions, leveraging its fluorescence for real-time monitoring.
These applications make it a versatile tool in research, diagnostics, and biotechnology.
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