This chemical is widely used as a fluorescent dye in biological and biochemical research. It is particularly valuable for measuring intracellular pH due to its pH-sensitive fluorescence properties. The dye is often employed in cell viability assays, where it helps distinguish between live and dead cells based on their pH levels. Additionally, it is utilized in fluorescence microscopy and flow cytometry to study cellular processes and monitor changes in pH within organelles. Its ability to permeate cell membranes makes it a useful tool for tracking cellular dynamics and responses to environmental changes.