Used primarily as a fluorescent tracer in various scientific applications, it is widely employed in biological and chemical research to detect and measure reactive oxygen species (ROS) within cells. Its ability to fluoresce upon oxidation makes it a valuable tool in studying oxidative stress and cellular damage. Additionally, it is utilized in environmental monitoring to track water flow and contamination in hydrological studies. In analytical chemistry, it serves as a pH indicator and a dye in fluorescence microscopy, aiding in the visualization of cellular structures and processes. Its high sensitivity and specificity make it a versatile compound in both research and industrial settings.