Used primarily as a fluorescent dye in biological and chemical research, it is widely applied in cell biology for detecting and measuring reactive oxygen species (ROS) within cells. It serves as an indicator in assays to study oxidative stress and cellular metabolism. In analytical chemistry, it is utilized as a pH indicator and tracer in chromatography due to its distinct fluorescence properties. Additionally, it finds application in microscopy for staining and visualizing cellular components, aiding in the study of cellular processes and structures. Its high sensitivity and specificity make it a valuable tool in various scientific investigations.