Used as a pH-sensitive fluorescent probe in analytical chemistry, particularly in fluorescence-based assays where its emission intensity changes with protonation state. Its dinitro substitution enhances photostability and shifts absorption to longer wavelengths, making it suitable for biological imaging under visible light excitation. Also applied in ion detection systems, where metal ions quench or enhance fluorescence, enabling selective sensing. Commonly employed in microplate assays and flow cytometry due to strong signal response and compatibility with standard optical filters.