Used extensively as a fluorescent probe in biochemical and cellular studies, this compound is particularly valuable for labeling proteins, peptides, and other biomolecules. Its ability to form stable conjugates with amino groups makes it a preferred choice for tracking and visualizing biological processes in live cells and tissues. The compound’s fluorescence properties allow for sensitive detection in various assays, including flow cytometry, fluorescence microscopy, and high-throughput screening. It is also employed in studying enzyme activities, receptor-ligand interactions, and intracellular signaling pathways. Its cell-permeant nature enables efficient labeling of intracellular targets, providing insights into dynamic cellular events. Additionally, it is utilized in diagnostic applications for detecting specific biomarkers in clinical samples.