5-FITC-DBCO is widely used in bioorthogonal chemistry for labeling and imaging applications. Its DBCO group enables rapid and selective reactions with azide-containing molecules through strain-promoted alkyne-azide cycloaddition (SPAAC), while the FITC fluorophore allows for fluorescence detection. This makes it highly valuable in live-cell imaging, where it can label specific biomolecules without interfering with cellular processes. It is also employed in proteomics for tagging and tracking proteins, as well as in drug delivery systems to monitor the distribution and interaction of therapeutic agents. Additionally, 5-FITC-DBCO is utilized in diagnostic assays and flow cytometry for sensitive and specific detection of target molecules. Its dual functionality ensures efficient conjugation and visualization in various biological and chemical studies.